The High School Human Genome Project

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Blast IT!

Images from Videodiscovery

Dideoxynucleotides   Sequencing Results
Using Automated Sequencer Reading with four colors Loading Gels Vortex and microtube
Water bath and microcentrifuge Micropipets and icing DNA Denaturing DNA at 95 degrees C  
Icing the DNA / Marking Lanes Preparing the Vertical Gel TBE Buffer in top & bottom chambers Polyacrylamide Gel
Cleaning the lanes of Urea and air bubles Loading the Amplified DNA Student and Doctor at Work A, C, G & T Termination Mixes
Loading with thin tips 1800 Volts for 1.5 hours Micropipet at Work Dye becomes visible
Close up of loading into wells   Polyacrylamide Gels before running DNA in gels Separation of glass plates following running of DNA in gels
Time for multiple solutions Membrane soaking Drain and prepare for next solution Wash Solution I
Teamwork & Wash solution II Distilled Water membranes in trays UV crosslinker creates covalent bonds between DNA and membranes
Practice reading the sequences Working Hard   Line by line
Look at this! Membrane results    
     
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