Images from Videodiscovery
The High School Human Genome Project
To access lesson plans and activities for this unit, click here
High School Human Genome Project Home page
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Images from Videodiscovery |
Dideoxynucleotides | Sequencing Results | |
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| Using Automated Sequencer | Reading with four colors | Loading Gels | Vortex and microtube |
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| Water bath and microcentrifuge | Micropipets and icing DNA | Denaturing DNA at 95 degrees C | |
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| Icing the DNA / Marking Lanes | Preparing the Vertical Gel | TBE Buffer in top & bottom chambers | Polyacrylamide Gel |
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| Cleaning the lanes of Urea and air bubles | Loading the Amplified DNA | Student and Doctor at Work | A, C, G & T Termination Mixes |
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| Loading with thin tips | 1800 Volts for 1.5 hours | Micropipet at Work | Dye becomes visible |
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| Close up of loading into wells | Polyacrylamide Gels before running DNA in gels | Separation of glass plates following running of DNA in gels | |
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| Time for multiple solutions | Membrane soaking | Drain and prepare for next solution | Wash Solution I |
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| Teamwork & Wash solution II | Distilled Water | membranes in trays | UV crosslinker creates covalent bonds between DNA and membranes |
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| Practice reading the sequences | Working Hard | Line by line | |
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| Look at this! | Membrane results | ||
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